Neutron scattering and selective deuteration for elucidating how lipids regulate metabolon formation
- Contact person:
- Marite Cardenas
- Responsible at Malmö University:
- Marite Cardenas
- Tomas Laursen - Copenhagen University Martin Andersson - Chalmers Technical University
- Time frame:
- 01 January 2019 - 31 December 2022
- Research environment :
- Research subject:
About the project
Most pharmaceutical compounds, colors and flavors are isolated from plants today. These valuable compounds are biosynthetised by a series of enzyme complexes that work in a concerted action. The assembly of such enzyme complexes, also called metabolons, facilitates the direct transfer of substrate between sequential enzymes, thereby, enhancing substrate channeling and prevent leakage of toxic or labile intermediates and metabolic cross-talk.
Organic synthesis in the lab is sometimes not possible or too expensive
Extracting pure products from plats constitutes a great industrial and societial challenge, since they exist in low amounts and as a part of complex mixtures of other potentially toxic compounds. Organic synthesis in the lab is sometimes not possible or too expensive, therefore the bioengenieering of moss and microorganism to express biosynthetic pathways for large-scale production of such compounds has been proposed lately. Since little knowledge exists on the conditions required for metabiolon assembly and dissasembly, there is a risk of release of undesired intermediates due to imperfect enzymatic channeling.
How plants guide the assembly of multi-enzyme complexes
Here, we will dissect the mechanisms by which plants guide the assembly of multi-enzyme complexes. We will use synthetic biology and neutron scattering to map the lipid-protein interactions that control the formation of thedhurrin metabolon, as a model system. This will enable rational enzyme design for improving the organization and stability of metabolons in heterologous hosts to boost the production of bioactive compounds.